2XBZ
Multiple oligomeric forms of the Pseudomonas aeruginosa RetS sensor domain modulate accessibility to the ligand-binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-22 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 174.187, 174.187, 174.187 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.650 |
| R-factor | 0.19502 |
| Rwork | 0.191 |
| R-free | 0.23453 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.619 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.020 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.100 | 0.530 |
| Number of reflections | 13389 | |
| <I/σ(I)> | 27.5 | 7.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.5 | 21.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M AMMONIUM CHLORIDE PH 6.3 AND 20% PEG 3350 |
