2XBT
Structure of a scaffoldin carbohydrate-binding module family 3b from the cellulosome of Bacteroides cellulosolvens: Structural diversity and implications for carbohydrate binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-07 |
Detector | ADSC CCD |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 83.193, 83.193, 96.139 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.697 - 1.832 |
R-factor | 0.1963 |
Rwork | 0.191 |
R-free | 0.24160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nbc |
RMSD bond length | 0.007 |
RMSD bond angle | 1.091 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.080 | 0.540 |
Number of reflections | 15209 | |
<I/σ(I)> | 29.2 | 1.9 |
Completeness [%] | 98.3 | 97 |
Redundancy | 8.64 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 1.0 M AMMONIUM SULFATE, 0.1 M HEPES PH 7.0, 0.5% (W/V) POLYETHYLENE GLYCOL 8000 |