2XB0
DNA-binding domain from Saccharomyces cerevisiae chromatin- remodelling protein Chd1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.450, 91.120, 98.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.255 - 2.000 |
| R-factor | 0.2093 |
| Rwork | 0.205 |
| R-free | 0.24790 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.359 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.200 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.820 |
| Number of reflections | 22943 | |
| <I/σ(I)> | 18.1 | 2.5 |
| Completeness [%] | 97.5 | 95.9 |
| Redundancy | 9.7 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M TRIS, PH 8.5, 28% PEG 4K, 0.35 M MGCL2 |
