2XAM
Inositol 1,3,4,5,6-pentakisphosphate 2-kinase from A. thaliana in complex with ADP and IP6.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2008-07-07 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.124, 113.591, 142.478 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.736 - 2.200 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.26950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xal |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.149 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.240 | 2.320 |
| High resolution limit [Å] | 1.730 | 2.200 |
| Rmerge | 0.140 | 0.530 |
| Number of reflections | 48686 | |
| <I/σ(I)> | 3.84 | 1.36 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 7.05 | 5.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.9 | 22% PEG 3350, 100 MM BIS-TRIS PH 5.9, 2 MM MGCL2. PROTEIN WAS MIXED WITH 2 MM ADP AND 2 MM INOSITOL-6-P |
