2XA1
Crystal structure of trehalose synthase TreT from P.horikoshii (Seleno derivative)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 297 |
| Detector technology | CCD |
| Collection date | 2005-12-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.149, 63.705, 90.584 |
| Unit cell angles | 90.00, 98.96, 90.00 |
Refinement procedure
| Resolution | 29.600 - 2.470 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.26900 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4I |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.100 | 0.300 |
| Number of reflections | 59214 | |
| <I/σ(I)> | 14.28 | 2.68 |
| Completeness [%] | 97.6 | 90.3 |
| Redundancy | 4.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | MPEG 2K 21%, 0.18M AMMONIUM SULFATE, 0.1M SODIUM ACETATE BUFFER (PH4.6), pH 8.5 |
