2X9Z

STRUCTURE OF THE PILUS BACKBONE (RRGB) FROM STREPTOCOCCUS PNEUMONIAE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	C 1 2 1
Unit cell lengths	94.975, 46.635, 64.380
Unit cell angles	90.00, 115.49, 90.00

Refinement procedure

Resolution	31.040 - 1.300
R-factor	0.152
Rwork	0.150
R-free	0.17800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x9w
RMSD bond length	0.005
RMSD bond angle	1.033
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.170
High resolution limit [Å]	1.150	1.150
Rmerge	0.070	0.770
Number of reflections	93417
<I/σ(I)>	25.3	1
Completeness [%]	93.9	88.5
Redundancy	7.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1