2X9Y

STRUCTURE OF THE PILUS BACKBONE (RRGB) FROM STREPTOCOCCUS PNEUMONIAE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	C 2 2 21
Unit cell lengths	43.667, 157.745, 147.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.084 - 2.330
R-factor	0.2028
Rwork	0.200
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x9w
RMSD bond length	0.004
RMSD bond angle	0.764
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.340
Rmerge	0.200	0.700
Number of reflections	21514
<I/σ(I)>	8.7	1.34
Completeness [%]	96.3	86.3
Redundancy	6.3	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PROTEIN WAS CRYSTALLIZED FROM 30% PEG-4000 0.1M TRIS PH 8.5