2X9M
Hendra virus attachment glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.883, 84.775, 91.548 |
| Unit cell angles | 103.39, 99.63, 108.62 |
Refinement procedure
| Resolution | 44.200 - 2.900 |
| R-factor | 0.23507 |
| Rwork | 0.233 |
| R-free | 0.27273 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vwd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.124 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.170 | 0.450 |
| Number of reflections | 38664 | |
| <I/σ(I)> | 6.1 | 2.9 |
| Completeness [%] | 99.2 | 98 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 30% PEG 8000, 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM CACODYLATE BUFFER PH 6.5 |
