2X9I
Structure of the Mutant D105N of Phycoerythrobilin Synthase PebS from the Cyanophage P-SSM2 in complex with bound substrate Biliverdin IXA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-12-17 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 52.920, 70.140, 81.970 |
Unit cell angles | 109.38, 109.25, 92.56 |
Refinement procedure
Resolution | 43.010 - 2.200 |
R-factor | 0.1783 |
Rwork | 0.176 |
R-free | 0.21150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vgr |
RMSD bond length | 0.012 |
RMSD bond angle | 1.435 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.000 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.290 |
Number of reflections | 50826 | |
<I/σ(I)> | 10.56 | 3.73 |
Completeness [%] | 96.6 | 96.6 |
Redundancy | 2.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 170MM (NH4)2SO4, 20%PEG3350 |