2X9B
The filamentous phages fd and IF1 use different infection mechanisms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 106.836, 106.836, 75.320 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.720 - 2.920 |
R-factor | 0.245 |
Rwork | 0.243 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g3p |
RMSD bond length | 0.007 |
RMSD bond angle | 0.870 |
Data reduction software | XDS |
Phasing software | AMoRE |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.720 | 3.260 |
High resolution limit [Å] | 2.920 | 2.920 |
Rmerge | 0.100 | 0.680 |
Number of reflections | 5889 | |
<I/σ(I)> | 20.6 | 3.87 |
Completeness [%] | 99.7 | 100 |
Redundancy | 11.38 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 3 M NACL, 5 % MPD, 0.1 M CACL2, 0.1 M IMIDAZOL PH 6.5 |