2X9A
crystal structure of g3p from phage IF1 in complex with its coreceptor, the C-terminal domain of TolA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY |
| Synchrotron site | BESSY |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Spacegroup name | H 3 |
| Unit cell lengths | 94.515, 94.515, 132.111 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.217 - 2.470 |
| R-factor | 0.1834 |
| Rwork | 0.181 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tol |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.032 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.220 | 2.530 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.050 | 0.650 |
| Number of reflections | 15411 | |
| <I/σ(I)> | 21.02 | 2.17 |
| Completeness [%] | 97.5 | 89 |
| Redundancy | 4.25 | 4.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 0.1 M SODIUM CITRATE, 20 % PEG3350, 5 % MPD, PH 4.5 |
