2X8Y
Crystal structure of AnCE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-10 |
Detector | ADSC CCD |
Spacegroup name | H 3 |
Unit cell lengths | 173.096, 173.096, 101.566 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.270 - 1.900 |
R-factor | 0.19535 |
Rwork | 0.194 |
R-free | 0.22045 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j38 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.982 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.210 |
Number of reflections | 89453 | |
<I/σ(I)> | 14.7 | 6.4 |
Completeness [%] | 95.0 | 89 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.5 |