2X8X
Structure of the N-terminal domain of Omp85 from the Thermophilic Cyanobacterium Thermosynechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESERACH |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 158.180, 158.180, 158.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.410 - 1.970 |
| R-factor | 0.20193 |
| Rwork | 0.200 |
| R-free | 0.23837 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.066 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.000 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 21967 | |
| <I/σ(I)> | 28.66 | 3 |
| Completeness [%] | 90.9 | 97.7 |
| Redundancy | 42 | 44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8% PEG 4000, 0.8M LiCl, 0.1M Tris-HCl, pH 8.5 |
