2X8R
The structure of a family GH25 lysozyme from Aspergillus fumigatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.650, 111.720, 119.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.500 - 1.700 |
| R-factor | 0.17094 |
| Rwork | 0.169 |
| R-free | 0.20741 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jfx |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.487 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0056) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.570 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.370 |
| Number of reflections | 118667 | |
| <I/σ(I)> | 13 | 3 |
| Completeness [%] | 99.0 | 99.5 |
| Redundancy | 6.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 0.1 M MIB SYSTEM (MALONIC ACID, IMIDAZOLE, BORIC ACID SYSTEM) PH 4.0 AND 25% PEG 3350 |
