2X8L
Plasmodium falciparum lactate dehydrogenase apo structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-26 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 80.120, 85.410, 91.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.500 - 1.600 |
R-factor | 0.13726 |
Rwork | 0.136 |
R-free | 0.16740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t24 |
RMSD bond length | 0.023 |
RMSD bond angle | 1.743 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.500 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.090 | 0.390 |
Number of reflections | 41592 | |
<I/σ(I)> | 13.8 | 2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 275 | MPD, HEPES, IMIDAZOLE, PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 2 |