2X8A

Human Nuclear Valosin containing protein Like (NVL), C-terminal AAA- ATPase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-09
Detector	ADSC CCD
Spacegroup name	P 61
Unit cell lengths	77.989, 77.989, 86.038
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.850 - 2.600
R-factor	0.2141
Rwork	0.210
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	BUSTER-TNT (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.900	2.670
High resolution limit [Å]	2.600	2.600
Rmerge	0.070	0.840
Number of reflections	8823
<I/σ(I)>	29.5	2.4
Completeness [%]	96.0	62.2
Redundancy	20.7	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1