2X8A
Human Nuclear Valosin containing protein Like (NVL), C-terminal AAA- ATPase domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-09 |
Detector | ADSC CCD |
Spacegroup name | P 61 |
Unit cell lengths | 77.989, 77.989, 86.038 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.850 - 2.600 |
R-factor | 0.2141 |
Rwork | 0.210 |
R-free | 0.25030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.160 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER-TNT (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.900 | 2.670 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.070 | 0.840 |
Number of reflections | 8823 | |
<I/σ(I)> | 29.5 | 2.4 |
Completeness [%] | 96.0 | 62.2 |
Redundancy | 20.7 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |