2X7Z
Crystal Structure of the SAP97 PDZ2 I342W C378A mutant protein domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-21 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 47.905, 47.905, 123.287 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.690 - 2.000 |
| R-factor | 0.19576 |
| Rwork | 0.194 |
| R-free | 0.23236 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2awu |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.691 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.220 |
| Number of reflections | 10153 | |
| <I/σ(I)> | 31.5 | 15.4 |
| Completeness [%] | 96.6 | 96.8 |
| Redundancy | 20.4 | 20.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.4 | VAPOR DIFFUSION, 100MM TRIS-HCL PH 8.4, 2.4M AMMONIUM SULPHATE |
