2X7X
Fructose binding periplasmic domain of hybrid two component system BT1754
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2008-05-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 111.840, 111.840, 115.171 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.690 - 2.640 |
| R-factor | 0.18838 |
| Rwork | 0.186 |
| R-free | 0.23195 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ioy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.246 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.780 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.100 | 0.350 |
| Number of reflections | 23919 | |
| <I/σ(I)> | 12.4 | 5.6 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.7 M POTASSIUM/SODIUM PHOSPHATE, 0.1 M HEPES PH 8.0 |
