2X7R
Crystal structure of a late fusion intermediate of HIV-1 gp41
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 57.422, 57.422, 182.768 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.729 - 2.000 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aik |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.119 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.380 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 22184 | |
| <I/σ(I)> | 17.9 | 2.8 |
| Completeness [%] | 96.5 | 84 |
| Redundancy | 5.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1M CITRIC ACID PH 6, 60% MPD |
