2X7P
The Conserved Candida albicans CA3427 Gene Product Defines a New Family of Proteins Exhibiting the Generic Periplasmic Binding Protein Structural Fold
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Collection date | 2004-07-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979774, 0.979958 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.588, 66.849, 113.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.880 - 2.340 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.25600 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.001 |
RMSD bond angle | 0.385 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.880 | 2.470 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.080 | 0.310 |
Number of reflections | 13442 | |
<I/σ(I)> | 4.1 | 3 |
Completeness [%] | 99.1 | 99.1 |
Redundancy | 5.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 21 % PEG8000, CALCIUM ACETATE 0.2 M, TRIS 0.1 M, 30 % GLYCEROL PH 7.0 |