2X7I
Crystal structure of mevalonate kinase from methicillin-resistant Staphylococcus aureus MRSA252
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 94.589, 94.589, 169.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.920 - 2.200 |
| R-factor | 0.21335 |
| Rwork | 0.211 |
| R-free | 0.25730 |
| Structure solution method | MIR |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.070 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.920 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 17784 | |
| <I/σ(I)> | 14.6 | 2.1 |
| Completeness [%] | 80.3 | 50.2 |
| Redundancy | 5.6 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 0.1 M CITRIC ACID PH 4.0, 3.6 M SODIUM CHLORIDE. THE CRYSTALS WERE CRYOPROTECTED BY ADDING 25% PEG400 INTO THE SCREEN SOLUTION. |
