2X7G

Structure of human serine-arginine-rich protein-specific kinase 2 (SRPK2) bound to purvalanol B

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-28
Detector	RAYONIX
Spacegroup name	P 31 2 1
格子定数 [Å]	110.310, 110.310, 90.590
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	45.000 - 2.500
R因子	0.18615
Rwork	0.183
R-free	0.24868
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wbp
結合長の平均二乗偏差(RMSD) [Å]	0.013
結合角の平均二乗偏差(RMSD) [度]	1.368
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	45.210	2.640
分解能 [Å] (高)	2.500	2.500
Rmerge_l_obs	0.130	0.590
独立反射数	22306
<I/σ(I)>	6.5	2
完全性 [%]	99.7	99.6
冗長性	3.2	3.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		4.75		0.5M AMMONIUM SULPHATE, 0.1M SODIUM ACETATE PH4.75