2X7B
Crystal structure of the N-terminal acetylase Ard1 from Sulfolobus solfataricus P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-03 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.440, 34.610, 49.830 |
| Unit cell angles | 90.00, 98.00, 90.00 |
Refinement procedure
| Resolution | 27.840 - 1.950 |
| R-factor | 0.20194 |
| Rwork | 0.200 |
| R-free | 0.25009 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ob0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.426 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.840 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.050 | 0.220 |
| Number of reflections | 11101 | |
| <I/σ(I)> | 19.5 | 8.8 |
| Completeness [%] | 91.2 | 93.8 |
| Redundancy | 3.78 | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25% PEG3350, 0.1M BIS-TRIS PH 5.5, 0.2 M NACL |
