2X77
Crystal Structure of Leishmania major ADP ribosylation factor-like 1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-05-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.286, 78.944, 58.034 |
| Unit cell angles | 90.00, 93.10, 90.00 |
Refinement procedure
| Resolution | 78.940 - 2.100 |
| R-factor | 0.21773 |
| Rwork | 0.214 |
| R-free | 0.28682 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1moz |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.815 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.100 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.490 |
| Number of reflections | 18516 | |
| <I/σ(I)> | 11.5 | 2.4 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | HANGING DROPS OF 0.75 UL PROTEIN SOLUTION, 0.75 UL 75 MM CALCIUM ACETATE, 17 % PEG 3350. |
