2X6P
Crystal Structure of Coil Ser L19C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 38.089, 38.089, 178.798 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.990 - 2.150 |
| R-factor | 0.22194 |
| Rwork | 0.220 |
| R-free | 0.26432 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1coi |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.318 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.990 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | |
| Number of reflections | 8556 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 97.3 | 89.1 |
| Redundancy | 8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100MM MES PH 6.5 40% PEG 200 |
