2X6C

Potassium Channel from Magnetospirillum Magnetotacticum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-04
Detector	ADSC CCD
Spacegroup name	P 4 21 2
Unit cell lengths	106.518, 106.518, 88.854
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.983 - 2.700
R-factor	0.2208
Rwork	0.219
R-free	0.26090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wlh
RMSD bond length	0.014
RMSD bond angle	1.250
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.080	0.930
Number of reflections	14682
<I/σ(I)>	23.2	2.3
Completeness [%]	99.9	100
Redundancy	7	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		AS PER WWPDB ENTRY 2X6A, EXCEPT THE CRYSTAL WAS SOAKED WITH 2MM SMAC (FINAL CONCENTRATION IN THE DROP) FOR 16HRS PRIOR TO DATA COLLECTION., pH 7.5