2X6B
Potassium Channel from Magnetospirillum Magnetotacticum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-16 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 104.798, 104.798, 88.417 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.409 - 3.300 |
| R-factor | 0.2368 |
| Rwork | 0.234 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wlh |
| RMSD bond length | 0.028 |
| RMSD bond angle | 1.019 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.110 | 0.870 |
| Number of reflections | 14195 | |
| <I/σ(I)> | 13.1 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | THE CRYSTAL WAS OBTAINED UNDER THE SAME CONDITIONS AS PDB ENTRY 2X6A, BUT SOAKED WITH BARIUM CHLORIDE (5MM FINAL CONCENTRATION) FOR 16HRS PRIOR TO CRYOCOOLING AND DATA COLLECTION, pH 7.5 |
