2X6A
Potassium Channel from Magnetospirillum Magnetotacticum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-16 |
Detector | ADSC CCD |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 107.759, 107.759, 89.242 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.621 - 3.100 |
R-factor | 0.2226 |
Rwork | 0.220 |
R-free | 0.27130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wlh |
RMSD bond length | 0.014 |
RMSD bond angle | 1.108 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.140 | 0.960 |
Number of reflections | 10032 | |
<I/σ(I)> | 20.1 | 2.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.8 | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | SITTING DROP VAPOUR DIFFUSION, 2:1 PROTEIN:CRYSTALLANT RATIO. CRYSTALLANT: 25% PEG400 2.5% PEG4K 2.5% PEG8K 10% GLYCEROL 90MM HEPES PH 7.5 ADDITIVES: FOS-CHOLINE-ISO-11-6U (ANATRACE) NONYL MALTOSIDE (ANATRACE) FOS-CHOLINE ISO-9 (ANATRACE) |