2X61
Crystal structure of the sialyltransferase CST-II in complex with trisaccharide acceptor and CMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 |
| Unit cell lengths | 117.665, 117.665, 46.604 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.950 |
| R-factor | 0.16553 |
| Rwork | 0.164 |
| R-free | 0.19752 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ro8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.068 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 46782 | |
| <I/σ(I)> | 38.3 | 5 |
| Completeness [%] | 99.6 | 96.9 |
| Redundancy | 6.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM HEPES5, PH 7.5, 8% (W/V) POLYETHYLENE GLYCOL 6000, AND 5% (V/V) 2-METHYL-2, 4-PENTANEDIOL |
