2X5V
80 microsecond laue diffraction snapshot from crystals of a photosynthetic reaction centre 3 millisecond following photoactivation.
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID09 |
Synchrotron site | ESRF |
Beamline | ID09 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2007-09-12 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8-1.1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 85.693, 143.470, 177.998 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.180 - 3.000 |
R-factor | 0.27866 |
Rwork | 0.277 |
R-free | 0.31940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wjn |
RMSD bond length | 0.014 |
RMSD bond angle | 1.786 |
Data reduction software | Precognition |
Data scaling software | Epinorm |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.200 | 3.140 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.180 | |
Number of reflections | 40530 | |
<I/σ(I)> | 10.4 | 6.9 |
Completeness [%] | 90.6 | 74.2 |
Redundancy | 7.7 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | MONOOLEIN WAS MIXED WITH 0.1M HEPES PH 8.1, 0.1% LDAO IN 60:40 (W/W) RATIO. THEN THE CUBIC PHASE WAS MIXED WITH 16% JEFFAMINE M600, 1M HEPES PH 8.1, 0.7M AMMONIUM SULFATE, 2.5% 1,2,3-HEPTANETRIOL IN 1 TO 4 RATIO AND EQUILIBRATED UNTIL PHASE SEPARATION OCCURED. THE UPPER LIPIDIC-SPONGE PHASE WAS HARVESTED AND 1UL WAS USED AS A PRECIPITANT SOLUTION TOGETHER WITH 1UL OF 25 MG/ML PROTEIN SOLUTION IN A HANGING-DROP, VAPOUR-DIFFUSION EXPERIMENT. |