2X5U
80 microsecond Laue diffraction snapshot from crystals of a photosynthetic reaction centre without illumination.
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID09 |
| Synchrotron site | ESRF |
| Beamline | ID09 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2007-09-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8-1.1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.693, 143.470, 177.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.780 - 3.000 |
| R-factor | 0.25167 |
| Rwork | 0.250 |
| R-free | 0.28434 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wjn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.750 |
| Data reduction software | Precognition |
| Data scaling software | Epinorm |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.800 | 3.140 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.150 | |
| Number of reflections | 40855 | |
| <I/σ(I)> | 12.9 | 8.6 |
| Completeness [%] | 91.3 | 75.9 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | MONOOLEIN WAS MIXED WITH 0.1M HEPES PH 8.1, 0.1% LDAO IN 60:40 (W/W) RATIO. THEN THE CUBIC PHASE WAS MIXED WITH 16% JEFFAMINE M600, 1M HEPES PH 8.1, 0.7M AMMONIUM SULFATE, 2.5% 1,2,3-HEPTANETRIOL IN 1 TO 4 RATIO AND EQUILIBRATED UNTIL PHASE SEPARATION OCCURED. THE UPPER LIPIDIC-SPONGE PHASE WAS HARVESTED AND 1UL WAS USED AS A PRECIPITANT SOLUTION TOGETHER WITH 1UL OF 25 MG/ML PROTEIN SOLUTION IN A HANGING-DROP, VAPOUR-DIFFUSION EXPERIMENT. |
