2X5T
Crystal structure of ORF131 from Sulfolobus islandicus rudivirus 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 57.810, 57.810, 69.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.060 - 2.200 |
| R-factor | 0.22379 |
| Rwork | 0.221 |
| R-free | 0.27493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x5g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.610 |
| Number of reflections | 6828 | |
| <I/σ(I)> | 25.5 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.8 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.1 M SODIUM MALONATE AND CRYOPROTECTED WITH 2.4 M MALONATE |
