2X5N
Crystal Structure of the SpRpn10 VWA domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-22 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.990, 35.230, 60.140 |
| Unit cell angles | 90.00, 111.20, 90.00 |
Refinement procedure
| Resolution | 27.738 - 1.300 |
| R-factor | 0.1248 |
| Rwork | 0.123 |
| R-free | 0.16460 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.587 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.100 | 1.400 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.040 | 0.160 |
| Number of reflections | 38709 | |
| <I/σ(I)> | 19.37 | 7.34 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS/HCL PH 8.5, 1.5 M (NH4)2SO4, 25 MM MGSO4, |
