2X5H
Crystal structure of the ORF131 L26M L51M double mutant from Sulfolobus islandicus rudivirus 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.940, 46.971, 48.976 |
| Unit cell angles | 90.22, 95.05, 91.78 |
Refinement procedure
| Resolution | 14.000 - 1.800 |
| R-factor | 0.20282 |
| Rwork | 0.200 |
| R-free | 0.25715 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x5g |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.149 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.190 |
| Number of reflections | 28543 | |
| <I/σ(I)> | 24.8 | 6.5 |
| Completeness [%] | 97.0 | 95 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.1 M SODIUM MALONATE AND CRYOPROTECTED WITH 2.4 M MALONATE. |
