2X5F
Crystal structure of the methicillin-resistant Staphylococcus aureus Sar2028, an aspartate_tyrosine_phenylalanine pyridoxal-5'-phosphate dependent aminotransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.780, 89.990, 104.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.240 - 1.800 |
| R-factor | 0.19198 |
| Rwork | 0.191 |
| R-free | 0.21049 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.196 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCDE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.240 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.120 | 0.540 |
| Number of reflections | 69033 | |
| <I/σ(I)> | 9.2 | 3.01 |
| Completeness [%] | 99.3 | 97 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 28% PEG3350, 0.1M HEPES PH 7.5. THE CRYSTALS WERE CRYOPROTECTED WITH 16% PEG400 |
