2X5D
Crystal Structure of a probable aminotransferase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.860, 173.810, 76.970 |
| Unit cell angles | 90.00, 114.51, 90.00 |
Refinement procedure
| Resolution | 28.890 - 2.250 |
| R-factor | 0.21753 |
| Rwork | 0.216 |
| R-free | 0.24422 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dou |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.357 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.740 |
| Number of reflections | 66956 | |
| <I/σ(I)> | 21.5 | 2.92 |
| Completeness [%] | 99.2 | 97.7 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 35.9% PEG400, 0.19M LITHIUM SULFATE, 0.1M MES PH6.0. CRYSTAL WAS CRYOPROTECTED DIRECTLY IN THIS SOLUTION SUPPLEMENTED WITH PLP. |
