2X5C
Crystal structure of hypothetical protein ORF131 from Pyrobaculum Spherical Virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-12-11 |
Detector | MARRESEARCH |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 53.880, 53.880, 127.459 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.824 - 1.800 |
R-factor | 0.1935 |
Rwork | 0.192 |
R-free | 0.22680 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.018 |
RMSD bond angle | 1.543 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXCDE (SOLVE RESOLVE) |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.050 | 0.480 |
Number of reflections | 19587 | |
<I/σ(I)> | 26.8 | 2.68 |
Completeness [%] | 99.0 | 89 |
Redundancy | 5.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 6.545% ISOPROPANOL, 0.1M TRIS-HCL, PH8.5. THE CRYOPROTECTANT USED WAS 30% GLYCEROL |