2X55

Yersinia Pestis Plasminogen Activator Pla (Native)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 65 2 2
Unit cell lengths	140.341, 140.341, 130.426
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.980 - 1.850
R-factor	0.196
Rwork	0.196
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NATIVE PLA OSMIUM DERIVATIVE
RMSD bond length	0.007
RMSD bond angle	1.240
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.070
Number of reflections	60190
<I/σ(I)>	46.1	3.1
Completeness [%]	100.0	100
Redundancy	12.1	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.8		27% PEG 400, 0.1 M LISO4, 0.1 M LITHIUM CITRATE, PH 4.8