2X4W
Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 43.261, 71.053, 113.982 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.505 - 1.500 |
R-factor | 0.1907 |
Rwork | 0.189 |
R-free | 0.21570 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.355 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.530 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.070 | 0.370 |
Number of reflections | 28447 | |
<I/σ(I)> | 14.9 | 3.6 |
Completeness [%] | 99.6 | 99.3 |
Redundancy | 5.4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 4M SODIUM FORMATE, 0.1M TRIS (PH 8.5), 0.01M NACL |