2X4S
Crystal structure of MHC CLass I HLA-A2.1 bound to a peptide representing the epitope of the H5N1 (Avian Flu) Nucleoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-04-13 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.377, 84.112, 80.035 |
Unit cell angles | 90.00, 90.97, 90.00 |
Refinement procedure
Resolution | 19.926 - 2.550 |
R-factor | 0.1818 |
Rwork | 0.178 |
R-free | 0.24750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eey |
RMSD bond length | 0.006 |
RMSD bond angle | 1.121 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.080 | 0.510 |
Number of reflections | 27072 | |
<I/σ(I)> | 5.8 | 1.1 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |