2X4J
Crystal structure of ORF137 from Pyrobaculum spherical virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-02-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | H 3 |
| Unit cell lengths | 89.770, 89.770, 36.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.900 - 1.620 |
| R-factor | 0.16964 |
| Rwork | 0.168 |
| R-free | 0.20241 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.158 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCDE (SOLVE RESOLVE) |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 1.740 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.080 | 0.570 |
| Number of reflections | 22381 | |
| <I/σ(I)> | 17.2 | 2.7 |
| Completeness [%] | 97.6 | 90.1 |
| Redundancy | 10.4 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 23% PEG3350, 0.37M MA-SCN, 0.23M MGCL2, 0.1M MES, PH 7.0. CRYSTAL CRYOPROTECTED WITH 40% PEG400 |
