2X3Y
Crystal structure of GmhA from Burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.095, 69.091, 128.148 |
| Unit cell angles | 98.35, 89.96, 104.19 |
Refinement procedure
| Resolution | 45.627 - 2.400 |
| R-factor | 0.1909 |
| Rwork | 0.189 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tk9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.955 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.720 |
| Number of reflections | 56237 | |
| <I/σ(I)> | 11.4 | 2.1 |
| Completeness [%] | 92.9 | 93.1 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 0.1 M SODIUM MALONATE PH 7.0, 10 % (W/V) PEG 3350. MICROBATCH METHOD |
