2X3V
Structure of The F-BAR Domain of Mouse Syndapin I
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.060, 153.490, 213.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.700 - 2.450 |
| R-factor | 0.21402 |
| Rwork | 0.212 |
| R-free | 0.26026 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.929 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | XPREP |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.750 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.080 | 0.840 |
| Number of reflections | 50261 | |
| <I/σ(I)> | 16.35 | 1.84 |
| Completeness [%] | 97.2 | 81.8 |
| Redundancy | 6.69 | 3.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 2UL SYNDAPIN-I/EHD-1(401 -534)(25MG/ML IN 50MM HEPES,200MM NACL) MIXED WITH WELL SOLUTION 0.1M SODIUM CITRATE BUFFER (PH5.5),0.2M NAAC,10%(W/V) PEG4000. ONLY F-BAR DOMAIN OF SYDAPIN I CRYSTALLIZED. |
