2X3T
Glutaraldehyde-crosslinked wheat germ agglutinin isolectin 1 crystal soaked with a synthetic glycopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.640, 93.210, 92.500 |
| Unit cell angles | 90.00, 98.32, 90.00 |
Refinement procedure
| Resolution | 32.653 - 2.749 |
| R-factor | 0.2315 |
| Rwork | 0.229 |
| R-free | 0.28470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wga |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.721 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.910 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.100 | 0.490 |
| Number of reflections | 19531 | |
| <I/σ(I)> | 12.1 | 3.45 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 3.8 | 3.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.9 | 0.04M KH2PO4, 20% GLYCEROL, 14%-17% PEG 8000, pH 4.9 |
