2X3H
COLIPHAGE K5A LYASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.530, 130.400, 145.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.180 - 1.600 |
| R-factor | 0.17779 |
| Rwork | 0.176 |
| R-free | 0.20349 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.186 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.360 |
| Number of reflections | 173321 | |
| <I/σ(I)> | 21.6 | 7.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | CRYSTALS WERE GROWN FROM HANGING DROPS; DROPS WERE FORMED BY MIXING 1UL OF 5.2 MG/ML HIS-KFLA IN 20MM TRISHCL (PH 8.0), 50MM NACL, 5% (V/V) GLYCEROL WITH AN EQUAL VOLUME OF WELL SOLUTION CONTAINING 0.2M KBR AND 10% PEG 3350. CRYSTALLIZATION WAS CARRIED OUT AT 20OC. |
