2X3A
AsaP1 inactive mutant E294Q, an extracellular toxic zinc metalloendopeptidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2009-02-26 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 98.249, 74.468, 54.703 |
Unit cell angles | 90.00, 112.35, 90.00 |
Refinement procedure
Resolution | 57.600 - 2.000 |
R-factor | 0.17203 |
Rwork | 0.170 |
R-free | 0.20461 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x3c |
RMSD bond length | 0.023 |
RMSD bond angle | 1.928 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.100 | 0.390 |
Number of reflections | 24682 | |
<I/σ(I)> | 18.17 | 3.3 |
Completeness [%] | 98.9 | 86.3 |
Redundancy | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.2 M MES PH 7.5, 1.6 M (NH4)2SO4 |