2X35
Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 44.214, 62.399, 114.958 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.421 - 2.000 |
R-factor | 0.1996 |
Rwork | 0.198 |
R-free | 0.23590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.284 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.300 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.294 |
Number of reflections | 10661 | |
<I/σ(I)> | 12.4 | 4.2 |
Completeness [%] | 96.5 | 91 |
Redundancy | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 20% PEG 3350, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5), 10 MM NACL |