2X34
Structure of a polyisoprenoid binding domain from Saccharophagus degradans implicated in plant cell wall breakdown
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2008-07-23 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.979, 64.964, 84.191 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.430 - 1.900 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.22300 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.479 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.410 |
| Number of reflections | 27856 | |
| <I/σ(I)> | 14.4 | 5.5 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 42% PEG600, 0.2M IMIDAZOL-MALATE PH 5.5 |
