2X2J
Crystal structure of the Gracilariopsis lemaneiformis alpha- 1,4-glucan lyase with deoxynojirimycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-01 |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.314, 91.707, 192.899 |
| Unit cell angles | 90.00, 99.33, 90.00 |
Refinement procedure
| Resolution | 33.190 - 2.350 |
| R-factor | 0.23673 |
| Rwork | 0.234 |
| R-free | 0.29121 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x2h |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.301 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.210 | 0.690 |
| Number of reflections | 175340 | |
| <I/σ(I)> | 4.3 | 1 |
| Completeness [%] | 91.0 | 85.2 |
| Redundancy | 4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 18-21% PEG 8000, 0.1 M SODIUM ACETATE, PH 5.0-5.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
