2X2H
Crystal structure of the Gracilariopsis lemaneiformis alpha-1,4- glucan lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 91.678, 96.629, 134.457 |
| Unit cell angles | 80.54, 83.28, 85.26 |
Refinement procedure
| Resolution | 132.450 - 2.060 |
| R-factor | 0.17068 |
| Rwork | 0.168 |
| R-free | 0.21453 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g3m |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.103 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.090 | 0.220 |
| Number of reflections | 258096 | |
| <I/σ(I)> | 8.14 | 2.9 |
| Completeness [%] | 93.2 | 76.4 |
| Redundancy | 2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 18-21% PEG 8000, 0.1 M SODIUM ACETATE, PH 5.0-5.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
